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《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.
Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO
《农业科学与工程前沿(英文)》 页码 302-313 doi: 10.15302/J-FASE-2021385
关键词: Citrus 2n gamete interploidy hybridization pericentromeric SNP marker second division restitution
Transcriptome resources and genome-wide marker development for Japanese larch (
Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG
《农业科学与工程前沿(英文)》 2014年 第1卷 第1期 页码 77-84 doi: 10.15302/J-FASE-2014010
关键词: Larix transcriptome age wood formation somatic embryogenesis molecular marker
Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency
Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang
《医学前沿(英文)》 2020年 第14卷 第3期 页码 335-356 doi: 10.1007/s11684-019-0705-9
关键词: traditional Chinese medicine Sijunzi decoction spleen qi deficiency syndrome Chinmedomics quality-marker
牙鲆50K SNP芯片的研制及其在抗病性状基因组选择中的应用 Article
周茜, 陈亚东, 卢昇, 刘洋, 徐文腾, 李仰真, 王磊, 王娜, 杨英明, 陈松林
《工程(英文)》 2021年 第7卷 第3期 页码 406-411 doi: 10.1016/j.eng.2020.06.017
null
《医学前沿(英文)》 2016年 第10卷 第1期 页码 52-60 doi: 10.1007/s11684-016-0433-3
Hepatocellular carcinoma (HCC) is a lethal liver malignancy worldwide. In this study, we reported that protein phosphatase magnesium-dependent 1δ (PPM1D) was highly expressed in the majority of HCC cases (approximately 59%) and significantly associated with high serum α-fetoprotein (AFP) level (P= 0.044). Kaplan-Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCC-specific overall survival (HR, 2.799; 95% CI, 1.346–5.818, P = 0.006). Overexpressing PPM1D promoted cell viability and invasion, whereas RNA interference-mediated knockdown of PPM1D inhibited proliferation, invasion, and migration of cultured HCC cells. In addition, PPM1D suppression by small interfering RNA decreased the tumorigenicity of HCC cells in vivo. Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target for HCC.
关键词: PPM1D hepatocellular carcinoma prognosis target therapy
Novel polymorphic EST-based microsatellite marker isolation and characterization from
Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG
《农业科学与工程前沿(英文)》 2015年 第2卷 第1期 页码 60-65 doi: 10.15302/J-FASE-2015048
Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,
《工程(英文)》 2022年 第18卷 第11期 页码 259-259 doi: 10.1016/j.eng.2022.09.002
关键词: online THE AUTHORS.Published repertoire behalf Engineering September genotyping Academy HD-marker approach” Education Press Limited Company.
原子界面催化合成SnP/CoP异质纳米晶嵌入碳杂化物用于高功率型锂离子电池 Article
胡晨, 胡彦杰, 陈爱平, 段学志, 江浩, 李春忠
《工程(英文)》 2022年 第18卷 第11期 页码 154-160 doi: 10.1016/j.eng.2021.11.026
磷化锡(SnP)具有极佳的锂离子扩散能力和高理论比容量,是高功率锂离子电池的理想负极材料。然而,SnP的合成难度高,大尺寸晶粒导致的电化学不可逆也阻碍了其应用。根据密度泛函理论(DFT)计算,使用原位催化磷化方法可以显著降低SnP的相对生成能。因此,在还原氧化石墨烯(rGO)包裹的碳骨架内合成了SnP/CoP异质纳米晶。充放电机理分析表明尺寸为4.0 nm的SnP/CoP纳米晶具有高反应可逆性,且CoP在较高电位生成的金属Co加速了低电位SnP反应的动力学,从而赋予材料超快充放电能力。
Detection of genomic signatures for pig hairlessness using high-density SNP data
Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING
《农业科学与工程前沿(英文)》 2014年 第1卷 第4期 页码 307-313 doi: 10.15302/J-FASE-2014039
Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS
《农业科学与工程前沿(英文)》 2019年 第6卷 第3期 页码 296-308 doi: 10.15302/J-FASE-2019269
The application of spectral reflectance indices (SRIs) as proxies to screen for yield potential (YP) and heat stress (HS) is emerging in crop breeding programs. Thus, a comparison of SRIs and their associations with grain yield (GY) under YP and HS conditions is important. In this study, we assessed the usefulness of 27 SRIs for indirect selection for agronomic traits by evaluating an elite spring wheat association mapping initiative (WAMI) population comprising 287 elite lines under YP and HS conditions. Genetic and phenotypic analysis identified 11 and 9 SRIs in different developmental stages as efficient indirect selection indices for yield in YP and HS conditions, respectively. We identified enhanced vegetation index (EVI) as the common SRI associated with GY under YP at booting, heading and late heading stages, whereas photochemical reflectance index (PRI) and normalized difference vegetation index (NDVI) were the common SRIs under booting and heading stages in HS. Genome-wide association study (GWAS) using 18704 single nucleotide polymorphisms (SNPs) from Illumina iSelect 90K identified 280 and 43 marker-trait associations for efficient SRIs at different developmental stages under YP and HS, respectively. Common genomic regions for multiple SRIs were identified in 14 regions in 9 chromosomes: 1B (60–62 cM), 3A (15, 85–90, 101–105 cM), 3B (132–134 cM), 4A (47–51 cM), 4B (71–75 cM), 5A (43–49, 56–60, 89–93 cM), 5B (124–125 cM), 6A (80–85 cM), and 6B (57–59, 71 cM). Among them, SNPs in chromosome 5A (89–93 cM) and 6A (80–85 cM) were co-located for yield and yield related traits. Overall, this study highlights the utility of SRIs as proxies for GY under YP and HS. High heritability estimates and identification of marker-trait associations indicate that SRIs are useful tools for understanding the genetic basis of agronomic and physiological traits.
关键词: genome-wide association study (GWAS) heat tolerance spectral reflectance spring wheat
A genome-wide association study of five meat quality traits in Yorkshire pigs
Qian DONG,Huiying LIU,Xinyun LI,Wei WEI,Shuhong ZHAO,Jianhua CAO
《农业科学与工程前沿(英文)》 2014年 第1卷 第2期 页码 137-143 doi: 10.15302/J-FASE-2014014
关键词: Pig GWAS meat quality trait SNP
The genetic regulation of skeletal muscle development: insights from chicken studies
Wen LUO, Bahareldin A. ABDALLA, Qinghua NIE, Xiquan ZHANG
《农业科学与工程前沿(英文)》 2017年 第4卷 第3期 页码 295-304 doi: 10.15302/J-FASE-2017159
关键词: chicken epigenetic modification miRNAs skeletal muscle development SNP transcription factor
The microRNA, miR-29c, participates in muscle development through targeting the
Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI
《农业科学与工程前沿(英文)》 2015年 第2卷 第4期 页码 311-317 doi: 10.15302/J-FASE-2015075
Genome-wide association study of the backfat thickness trait in two pig populations
Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN
《农业科学与工程前沿(英文)》 2014年 第1卷 第2期 页码 91-95 doi: 10.15302/J-FASE-2014005
关键词: backfat thickness SNP chip genome-wide association study compressed mixed linear model pig
标题 作者 时间 类型 操作
UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)
期刊论文
UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (
Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO
期刊论文
Transcriptome resources and genome-wide marker development for Japanese larch (
Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG
期刊论文
Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency
Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang
期刊论文
Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma
null
期刊论文
Novel polymorphic EST-based microsatellite marker isolation and characterization from
Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG
期刊论文
Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker
Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,
期刊论文
Detection of genomic signatures for pig hairlessness using high-density SNP data
Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING
期刊论文
as proxies for yield potential and heat stress tolerance in spring wheat: heritability estimates and marker-trait
Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS
期刊论文
A genome-wide association study of five meat quality traits in Yorkshire pigs
Qian DONG,Huiying LIU,Xinyun LI,Wei WEI,Shuhong ZHAO,Jianhua CAO
期刊论文
The genetic regulation of skeletal muscle development: insights from chicken studies
Wen LUO, Bahareldin A. ABDALLA, Qinghua NIE, Xiquan ZHANG
期刊论文
The microRNA, miR-29c, participates in muscle development through targeting the
Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI
期刊论文